Sequencing:Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
Article Snippet: .. To avoid repeated Cas9-editing modifications, the added GBSs overlapped with the gRNA target sequence (genomic location of introduced GBSs listed in ). gRNAs for genome editing ( ) were designed using the CRISPOR webtool ( http://crispor.tefor.net/ ) and cloned into the sgRNA/Cas9 expression construct PX459 (#62988; Addgene). .. To generate clonal lines with HDR-induced sequence changes, U2OS-GR cells were transfected using 600 ng of the gRNA construct and 3 μg of the HDR template by nucleofection (Lonza Nucleofector kit V) according to the manufacturer’s instructions.
Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
Article Snippet: .. To avoid repeated Cas9-editing modifications, the added GBSs overlapped with the gRNA target sequence (genomic location of introduced GBSs listed in ). gRNAs for genome editing ( ) were designed using the CRISPOR webtool ( http://crispor.tefor.net/ ) and cloned into the sgRNA/Cas9 expression construct PX459 (Addgene #62988). .. To generate clonal lines with HDR-induced sequence changes, U2OS-GR cells were transfected using 600 ng of the gRNA construct and 3 μg of the HDR template by nucleofection (Lonza Nucleofector Kit V) according to the manufacturer’s instructions.
Clone Assay:Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
Article Snippet: .. To avoid repeated Cas9-editing modifications, the added GBSs overlapped with the gRNA target sequence (genomic location of introduced GBSs listed in ). gRNAs for genome editing ( ) were designed using the CRISPOR webtool ( http://crispor.tefor.net/ ) and cloned into the sgRNA/Cas9 expression construct PX459 (#62988; Addgene). .. To generate clonal lines with HDR-induced sequence changes, U2OS-GR cells were transfected using 600 ng of the gRNA construct and 3 μg of the HDR template by nucleofection (Lonza Nucleofector kit V) according to the manufacturer’s instructions.
Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
Article Snippet: .. To avoid repeated Cas9-editing modifications, the added GBSs overlapped with the gRNA target sequence (genomic location of introduced GBSs listed in ). gRNAs for genome editing ( ) were designed using the CRISPOR webtool ( http://crispor.tefor.net/ ) and cloned into the sgRNA/Cas9 expression construct PX459 (Addgene #62988). .. To generate clonal lines with HDR-induced sequence changes, U2OS-GR cells were transfected using 600 ng of the gRNA construct and 3 μg of the HDR template by nucleofection (Lonza Nucleofector Kit V) according to the manufacturer’s instructions.
Expressing:Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
Article Snippet: .. To avoid repeated Cas9-editing modifications, the added GBSs overlapped with the gRNA target sequence (genomic location of introduced GBSs listed in ). gRNAs for genome editing ( ) were designed using the CRISPOR webtool ( http://crispor.tefor.net/ ) and cloned into the sgRNA/Cas9 expression construct PX459 (#62988; Addgene). .. To generate clonal lines with HDR-induced sequence changes, U2OS-GR cells were transfected using 600 ng of the gRNA construct and 3 μg of the HDR template by nucleofection (Lonza Nucleofector kit V) according to the manufacturer’s instructions.
Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
Article Snippet: .. To avoid repeated Cas9-editing modifications, the added GBSs overlapped with the gRNA target sequence (genomic location of introduced GBSs listed in ). gRNAs for genome editing ( ) were designed using the CRISPOR webtool ( http://crispor.tefor.net/ ) and cloned into the sgRNA/Cas9 expression construct PX459 (Addgene #62988). .. To generate clonal lines with HDR-induced sequence changes, U2OS-GR cells were transfected using 600 ng of the gRNA construct and 3 μg of the HDR template by nucleofection (Lonza Nucleofector Kit V) according to the manufacturer’s instructions.
Construct:Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
Article Snippet: .. To avoid repeated Cas9-editing modifications, the added GBSs overlapped with the gRNA target sequence (genomic location of introduced GBSs listed in ). gRNAs for genome editing ( ) were designed using the CRISPOR webtool ( http://crispor.tefor.net/ ) and cloned into the sgRNA/Cas9 expression construct PX459 (#62988; Addgene). .. To generate clonal lines with HDR-induced sequence changes, U2OS-GR cells were transfected using 600 ng of the gRNA construct and 3 μg of the HDR template by nucleofection (Lonza Nucleofector kit V) according to the manufacturer’s instructions.
Article Title: Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
Article Snippet: .. To avoid repeated Cas9-editing modifications, the added GBSs overlapped with the gRNA target sequence (genomic location of introduced GBSs listed in ). gRNAs for genome editing ( ) were designed using the CRISPOR webtool ( http://crispor.tefor.net/ ) and cloned into the sgRNA/Cas9 expression construct PX459 (Addgene #62988). .. To generate clonal lines with HDR-induced sequence changes, U2OS-GR cells were transfected using 600 ng of the gRNA construct and 3 μg of the HDR template by nucleofection (Lonza Nucleofector Kit V) according to the manufacturer’s instructions.
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